LOCUS       40924_23714       11442 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB713 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 11442)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11442)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11442)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2083..2172
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      2206..2458
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2492..2508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2516..2532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2540..2570)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2585..2606)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2642..2894)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2932..4227)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61196.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(2932..4227)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4279..4458)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4964..4988)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5509..6297
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6546..7134
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7320..7460)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7804..7998)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8067..9137)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9569..10195)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcgggtta attaacatct tttacccata cgatgttcct
     2101 gactatgcgg gctatccgta tgacgtcccg gactatgcag gatcctatcc atatgacgtt
     2161 ccagattacg ctgctcagtg cagcgcttag agctcgaatt tccccgatcg ttcaaacatt
     2221 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2281 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2341 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     2401 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     2461 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2521 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2581 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tattaattcc
     2641 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     2701 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     2761 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     2821 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     2881 atgtttgaac gatcggggaa attcgcctcg agtgacttct ctgattgcag actatttata
     2941 ccaaccagca agaaagtgtc gaagtatgaa gcagaaggcg caggctagag cctggaaaac
     3001 gagaagatgg atgcaaagag acttttctga tttaggacca aacaacctca agtaaacatt
     3061 gacgagtgtc ccatcttttg tgccggtcag taatccggta gcaggatcat accttgggag
     3121 acgatggcgc ggacatttca caatcccagc aagctgagga agcgataata gaaggttcag
     3181 aacttgagga agaaagaaga tgaggagggt ttcactgaag tgacccagaa taccaacaac
     3241 tgccatagtc attccggcga agactgtgta agtgtctcca acaaaaactg aagaaggata
     3301 ccaattgtaa gcaagcattg ccaaggatgt ggccatcagc ggttgagtaa gaaatatcga
     3361 gaaggcatga gcttggtgat actcgggatc aacagatgct ccgatttgca taacgttgtg
     3421 tatcaggata gcagcggcaa taacaactgt ttgaccgatt tcaaggccat tcagaccagc
     3481 gtggatgtta atagagtttg tacaaaagac agcaagtagc cccatatata acttatatat
     3541 ccttcctaga ttaagcactt ccaagccaat ataagcaaca agaggttttg gtataacaat
     3601 agttgtatga ccagcataag ccatcaaaag tggaagggta gcaaaagatg gcaggacaag
     3661 ttttactctc caaggcacat caaggacatc atctacaaat ccaagcaaaa tcatgaagca
     3721 aatagatgct agtgctgcat tatactccac aagccaatta gaatcttcag tgaagttaaa
     3781 atactgaaat attatcgcca cgatcaaaaa gacaatacca acaacaatac ccaatgactc
     3841 tggcacttta atatctcctt gaggcgtgcc tcttttgttg atatcaaaac caaacatgtt
     3901 acgtctcaga acgtatctgg cagtaacagg aatcattttg agagtgacga aaaacccaac
     3961 caagctaagc cccgcgttaa ttagaatcga tcgcttgagt tcattatcaa ccttgtagtg
     4021 aaaacagaga aggtatagat agagcgaaca gagaagagta gagatcacga agatcactcc
     4081 taatttcggc ggcgcgagac gaaattcctc accggaagca gaaaccgtct ttcgggtcat
     4141 cttctgctcc gatggcgctg agttgggttc cgacgggtcc ggtttgttgg gaattgagat
     4201 cgatgaagct ctcttacgag ctgccatatc ttattgcccc ccgggatcag atctggattg
     4261 agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca
     4321 tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat
     4381 aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct
     4441 ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc
     4501 gggggtcata acgtgactcc cttaattctc cgctcatgat cttgatcccc tgcgccatca
     4561 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     4621 gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta
     4681 tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt
     4741 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     4801 ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc agcgtgaagc
     4861 tgatccgtcg agattaatag aaataaattc agcctaattc ggcgttaatt cagtacatta
     4921 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     4981 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     5041 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     5101 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     5161 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     5221 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     5281 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     5341 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     5401 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     5461 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
     5521 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     5581 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     5641 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     5701 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     5761 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     5821 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     5881 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     5941 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     6001 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     6061 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     6121 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     6181 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     6241 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     6301 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     6361 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     6421 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
     6481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6661 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7261 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7321 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     7381 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     7441 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     7501 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     7561 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
     7621 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     7681 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     7741 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     7801 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     7861 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     7921 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     7981 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
     8041 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
     8101 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
     8161 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
     8221 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
     8281 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
     8341 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
     8401 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
     8461 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
     8521 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
     8581 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
     8641 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
     8701 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
     8761 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
     8821 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
     8881 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
     8941 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
     9001 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
     9061 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
     9121 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
     9181 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
     9241 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
     9301 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
     9361 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
     9421 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
     9481 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
     9541 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
     9601 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
     9661 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
     9721 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
     9781 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
     9841 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
     9901 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
     9961 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    10021 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    10081 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    10141 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    10201 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    10261 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    10321 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    10381 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    10441 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    10501 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    10561 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    10621 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    10681 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    10741 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    10801 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    10861 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    10921 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    10981 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    11041 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    11101 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    11161 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    11221 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    11281 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    11341 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc
    11401 tgtggttggc atgcacatac aaatggacga acggataaac ct
//
